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Metabolically engineered E. coli producing phenol
Many chemicals we use in everyday life are derived from fossil resources. Due to the increasing concerns on the use of fossil resources, there has been much interest in producing chemicals from renewable resources through biotechnology.
Phenol is an important commodity chemical, and is a starting material for the production of numerous industrial chemicals and polymers, including bisphenol A and phenolic resins, and others. At present, the production of phenol entirely depends on the chemical synthesis from benzene, and its annual production exceeds 8 million tons worldwide. Microbial production of phenol seems to be a non-viable process considering the high toxicity of phenol to the cell.
In the paper published online in Biotechnology Journal, a Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) reported the successful development of an engineered Escherichia coli (E. coli) strain which can produce phenol from glucose. E. coli has been a workhorse for biological production of various value-added compounds such as succinic acid and 1,4-butanediol in industrial scale. However, due to its low tolerance to phenol, E. coli was not considered a viable host strain for the biological production of phenol.
Professor Lee"s team, a leading research group in metabolic engineering, noted the genetic and physiological differences of various E. coli strains and investigated 18 different E. coli strains with respect to phenol tolerance and engineered all of the 18 strains simultaneously. If the traditional genetic engineering methods were used, this work would have taken years to do. To overcome this challenge, the research team used synthetic small RNA (sRNA) technology they recently developed (Nature Biotechnology, vol 31, pp 170-174, 2013). The sRNA technology allowed the team to screen 18 E. coli strains with respect to the phenol tolerance, and the activities of the metabolic pathway and enzyme involved in the production of phenol. The research team also metabolically engineered the E. coli strains to increase carbon flux toward phenol and finally generated an engineered E. coli strain which can produce phenol from glucose.
Furthermore, the team developed a biphasic extractive fermentation process to minimize the toxicity of phenol to E. coli cells. Glycerol tributyrate was found to have low toxicity to E. coli and allowed efficient extraction of phenol from the culture broth. Through the biphasic fed-batch fermentation using glycerol tributyrate as an in situ extractant, the final engineered E. coli strain produced phenol to the highest titer and productivity reported (3.8 g/L and 0.18 g/L/h, respectively). The strategy used for the strain development and the fermentation process will serve as a framework for metabolic engineering of microorganisms for the production of toxic chemicals from renewable resources.
This work was supported by the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT & Future Planning through the National Research Foundation of Korea.
Process of Phenol Production
2013.11.05 View 10564 -
KAIST announced a novel technology to produce gasoline by a metabolically engineered microorganism
A major scientific breakthrough in the development of renewable energy sources and other important chemicals; The research team succeeded in producing 580 mg of gasoline per liter of cultured broth by converting in vivo generated fatty acids
For many decades, we have been relying on fossil resources to produce liquid fuels such as gasoline, diesel, and many industrial and consumer chemicals for daily use. However, increasing strains on natural resources as well as environmental issues including global warming have triggered a strong interest in developing sustainable ways to obtain fuels and chemicals.
Gasoline, the petroleum-derived product that is most widely used as a fuel for transportation, is a mixture of hydrocarbons, additives, and blending agents. The hydrocarbons, called alkanes, consist only of carbon and hydrogen atoms. Gasoline has a combination of straight-chain and branched-chain alkanes (hydrocarbons) consisted of 4-12 carbon atoms linked by direct carbon-carbon bonds.
Previously, through metabolic engineering of Escherichia coli (E. coli), there have been a few research results on the production of long-chain alkanes, which consist of 13-17 carbon atoms, suitable for replacing diesel. However, there has been no report on the microbial production of short-chain alkanes, a possible substitute for gasoline.
In the paper (entitled "Microbial Production of Short-chain Alkanes") published online in Nature on September 29, a Korean research team led by Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST) reported, for the first time, the development of a novel strategy for microbial gasoline production through metabolic engineering of E. coli.
The research team engineered the fatty acid metabolism to provide the fatty acid derivatives that are shorter than normal intracellular fatty acid metabolites, and introduced a novel synthetic pathway for the biosynthesis of short-chain alkanes. This allowed the development of platform E. coli strain capable of producing gasoline for the first time. Furthermore, this platform strain, if desired, can be modified to produce other products such as short-chain fatty esters and short-chain fatty alcohols.
In this paper, the Korean researchers described detailed strategies for 1) screening of enzymes associated with the production of fatty acids, 2) engineering of enzymes and fatty acid biosynthetic pathways to concentrate carbon flux towards the short-chain fatty acid production, and 3) converting short-chain fatty acids to their corresponding alkanes (gasoline) by introducing a novel synthetic pathway and optimization of culture conditions. Furthermore, the research team showed the possibility of producing fatty esters and alcohols by introducing responsible enzymes into the same platform strain.
Professor Sang Yup Lee said, "It is only the beginning of the work towards sustainable production of gasoline. The titer is rather low due to the low metabolic flux towards the formation of short-chain fatty acids and their derivatives. We are currently working on increasing the titer, yield and productivity of bio-gasoline. Nonetheless, we are pleased to report, for the first time, the production of gasoline through the metabolic engineering of E. coli, which we hope will serve as a basis for the metabolic engineering of microorganisms to produce fuels and chemicals from renewable resources."
This research was supported by the Advanced Biomass Research and Development Center of Korea (ABC-2010-0029799) through the Global Frontier Research Program of the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF), Republic of Korea. Systems metabolic engineering work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) by MSIP through NRF.
Short-Chain Alkanes Generated from Renewable Biomass
This diagram shows the metabolic engineering of Escherichia coli for the production of short-chain alkanes (gasoline) from renewable biomass.
Nature Cover Page (September 29th, 2013)
2013.11.04 View 12501 -
A powerful strategy for developing microbial cell factories by employing synthetic small RNAs
The current systems for the production of chemicals, fuels and materials heavily rely on the use of fossil resources. Due to the increasing concerns on climate change and other environmental problems, however, there has been much interest in developing biorefineries for the production of such chemicals, fuels and materials from renewable resources. For the biorefineries to be competitive with the traditional fossil resource-based refineries, development of high performance microorganisms is the most important as it will affect the overall economics of the process most significantly. Metabolic engineering, which can be defined as purposeful modification of cellular metabolic and regulatory networks with an aim to improve the production of a desired product, has been successfully employed to improve the performance of the cell. However, it is not trivial to engineer the cellular metabolism and regulatory circuits in the cell due to their high complexity.
In metabolic engineering, it is important to find the genes that need to be amplified and attenuated in order to increase the product formation rate while minimizing the production of undesirable byproducts. Gene knock-out experiments are often performed to delete those metabolic fluxes that will consequently result in the increase of the desired product formation. However, gene knock-out experiments require much effort and time to perform, and are difficult to do for a large number of genes. Furthermore, the gene knock-out experiments performed in one strain cannot be transferred to another organism and thus the whole experimental process has to be repeated. This is a big problem in developing a high performance microbial cell factory because it is required to find the best platform strain among many different strains. Therefore, researchers have been eager to develop a strategy that allows rapid identification of multiple genes to be attenuated in multiple strains at the same time.
A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) reported the development of a strategy for efficiently developing microbial cell factories by employing synthetic small RNAs (sRNAs). They first reported the development of such system in Nature Biotechnology last February. This strategy of employing synthetic sRNAs in metabolic engineering has been receiving great interest worldwide as it allows easy, rapid, high-throughput, tunable, and un-doable knock-down of multiple genes in multiple strains at the same time. The research team published a paper online on August 8 as a cover page (September issue) in Nature Protocols, describing the detailed strategy and protocol to employ synthetic sRNAs for metabolic engineering.
In this paper, researchers described the detailed step-by-step protocol for synthetic sRNA-based gene expression control, including the sRNA design principles. Tailor-made synthetic sRNAs can be easily manipulated by using conventional gene cloning method. The use of synthetic sRNAs for gene expression regulation provides several advantages such as portability, conditionality, and tunability in high-throughput experiments. Plasmid-based synthetic sRNA expression system does not leave any scar on the chromosome, and can be easily transferred to many other host strains to be examined. Thus, the construction of libraries and examination of different host strains are much easier than the conventional hard-coded gene manipulation systems. Also, the expression of genes can be conditionally repressed by controlling the production of synthetic sRNAs. Synthetic sRNAs possessing different repression efficiencies make it possible to finely tune the gene expression levels as well. Furthermore, synthetic sRNAs allow knock-down of the expression of essential genes, which was not possible by conventional gene knock-out experiments.
Synthetic sRNAs can be utilized for diverse experiments where gene expression regulation is needed. One of promising applications is high-throughput screening of the target genes to be manipulated and multiple strains simultaneously to enhance the production of chemicals and materials of interest. Such simultaneous optimization of gene targets and strains has been one of the big challenges in metabolic engineering. Another application is to fine tune the expression of the screened genes for flux optimization, which would enhance chemical production further by balancing the flux between biomass formation and target chemical production. Synthetic sRNAs can also be applied to finely regulating genetic interactions in a circuit or network, which is essential in synthetic biology. Once a sRNA scaffold-harboring plasmid is constructed, tailor-made, synthetic sRNAs can be made within 3-4 days, followed by the desired application experiments.
Dr. Eytan Zlotorynski, an editor at Nature Protocols, said "This paper describes the detailed protocol for the design and applications of synthetic sRNA. The method, which has many advantages, is likely to become common practice, and prove useful for metabolic engineering and synthetic biology studies."
This paper published in Nature Protocols will be useful for all researchers in academia and industry who are interested in the use of synthetic sRNAs for fundamental and applied biological and biotechnological studies.
This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) and the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea.
2013.10.31 View 10045 -
Therapy developed to induce Angiogenesis of Retina
- Junyeop Lee, Graduate School of Medical Sciences and Engineering
- Research results expected to be applied for treatment of diabetic retinopathy
A major clue to treatment of retinovascular disease, which causes blindness, has been found. The key to protection of the retinal nerve is the angiogenic protein that promotes healthy retinal vessel growth around the retina, which usually does not receive blood supply readily. This research offers a beginning to the possible improvement of therapy for diabetic retinopathy1 and retinopathy of prematurity2. Also important to the research is the fact that the ophthalmology specialist researcher, currently undergoing professional training, provided the results.
KAIST Graduate School of Medical Sciences and Engineering’s Junyeop Lee is the opthalmology specialist, who carried out the research under supervision by academic advisers Gyuyeong Go and Wookjun Yoo. The Ministry of Science, ICT and Future Planning as well as the National Research Foundation of Korea have funded his research. The research results have been published as a cover paper on ‘Science Translational Medicine’ on 18th August. This journal is a sister publication of Science, which is prestigious in the field of translational medicine that ties the basic science with clinical medicine. (Thesis title: Angiopoietin-1 Guides Directional Angiogenesis Through Integrin αvβ5 Signaling for Recovery of Ischemic Retinopathy)
The traditional treatment of diabetic retinopathy includes laser photocoagulation to destroy the retinal tissues or antibody therapeutics, which prevents vessel proliferation and blood leaking. The advantage of antibody therapeutics3 is that it retains the retinal nerves, however, it is not the fundamental solution but merely a temporary one, which requires repeated treatments.
The research team identified that Angiopoietin-14 protein, known as essential for growth and stabilization of vessels, also plays an important role in retinal vessel growth. The protein protects the retinal nerves, as well as provides improvement for retinal ischemia5 that is the root cause of vision loss due to retinal hemorrhages. It is expected to become a key to finding fundamental treatment method – by providing sufficient blood supply to the retina, thereby preserving the retinal nerve functions.
The results show that administration of Angiopoietin-1 to retinopathy mouse model promotes growth of healthy vessel growth, further preventing abnormal vessel growth, retinal hemorrhage and vision loss due to retinal ischemia.
Junyeop Lee said, “This research has identified that Angiopoietin-1 is an important factor in retinal vessel generation and stabilization. The paradigm will shift from traditional treatment method, which prevents vessel growth, to a new method that generates healthy vessels and strengthens vessel functions.”
1 Diabetic retinopathy: This retinovascular disease is a diabetic complication caused by insufficient blood supply. It is the major causes of blindness in adults.
2 Retinopathy of prematurity: The retinal vascular disease that occurs in premature infants with incomplete retinal vascular development. It is also the most common cause of blindness in children.
3 Antibody Therapeutics: Antibody developed to selectively inhibit abnormal blood vessel growth and leakage. Typical antibody therapeutics is Avastin and Lucentis, which hinder vascular endothelial growth factor (VEGF).
4 Angiopoietin-1: A critical growth factor that induces the production of healthy blood vessels and maintains the stability of the created vessel.
5 Retinal ischemia: State of ailment where retinal tissue blood supply is not sufficient.
Figure 1. Retinopathy mouse models show that, in comparison to the control group, the VEGF-Trap treatment and Angiopoietin-1 (Ang1) treatment groups significantly suppresses the pathological vascular proliferation. In addition, the Ang 1 group show vessel growth toward the central avascular area (region of retinal ischemia), which is not observed in VEGF-Trap treatment.
Figure 2. Reduced retinal ischemia, retinal bleeding and blood vessel normalization by Angiopoietin-1. Retinal ischemic region (arrow) and retinal bleeding significantly reduced in the Angiopoietin-1 (Ang1) treatment model in comparison to control group (left). The newly generated vessels in Ang 1 model are structurally supported by perivascular cells as normal retinal vessels do (right).
2013.10.12 View 11887 -
KAIST to establish Ombudsperson system
KAIST has recently undergone a massive reorganization to achieve a streamlined system and highly efficient administration; and it will now implement the new “Ombudsperson” system to hear the opinions of the members of the university.
On September 9th, President Sungmo Kang held a ceremony to appoint Professors Sang-Young Shin and Hong-Gu Shim as the new “Ombudspersons”.
The previous Shinmungo system raised complaints and recommendations for improvements by members of the university, but this is the first time that KAIST has assigned a direct department for handling such matters.
The newly appointed Ombudspersons will review for the possibility of any unjust, irrational systems, violations of research ethics and such. It is their role to take a neutral stance and advise on the correction and improvement.
The merit of the Ombudsperson system is that diverse opinions can be reflected on the policy. The Ombudsperson guarantees the security of the contents of discussion so that anyone can share his or her opinion without fear of being recorded in documents.
It is expected that the Ombudsperson system will protect the interests of the individuals and thus contribute to making a “happy campus”.
President [Sungmo] Kang has said that the reason establishing the office of the Ombudsperson is “In order for KAIST to take a new leap toward the world, it is crucial to bring the minds of the members together…. Even the smallest voices must be heard to present solutions to make the university where everyone’s happy.”
In 1809, the Swedish Parliament appointed the first “Ombudsperson” to investigate and resolve civil complaints. Now, it is widely used in public institutions, corporations and universities to improve the communication and work efficiency of the members.
The new Ombudsmen: Prof. Sang-Young Shin (left) and Prof. Hong-Gu Shim (right)
2013.09.27 View 11587 -
Ultra-High Strength Metamaterial Developed Using Graphene
New metamaterial has been developed, exhibiting hundreds of times greater strength than pure metals.
Professor Seung Min, Han and Yoo Sung, Jeong (Graduate School of Energy, Environment, Water, and Sustainability (EEWS)) and Professor Seok Woo, Jeon (Department of Material Science and Engineering) have developed a composite nanomaterial. The nanomaterial consists of graphene inserted in copper and nickel and exhibits strengths 500 times and 180 times, respectively, greater than that of pure metals. The result of the research was published on the July 2nd online edition in Nature Communications journal.
Graphene displays strengths 200 times greater than that of steel, is stretchable, and is flexible. The U.S. Army Armaments Research, Development and Engineering Center developed a graphene-metal nanomaterial but failed to drastically improve the strength of the material.
To maximize the strength increased by the addition of graphene, the KAIST research team created a layered structure of metal and graphene. Using CVD (Chemical Vapor Deposition), the team grew a single layer of graphene on a metal deposited substrate and then deposited another metal layer. They repeated this process to produce a metal-graphene multilayer composite material, utilizing a single layer of graphene. Micro-compression tests within Transmission Electronic Microscope and Molecular Dynamics simulations effectively showed the strength enhancing effect and the dislocation movement in grain boundaries of graphene on an atomic level.
The mechanical characteristics of the graphene layer within the metal-graphene composite material successfully blocked the dislocations and cracks from external damage from traveling inwards. Therefore the composite material displayed strength beyond conventional metal-metal multilayer materials. The copper-graphene multilayer material with an interplanar distance of 70nm exhibited 500 times greater (1.5GPa) strength than pure copper. Nickel-graphene multilayer material with an interplanar distance of 100nm showed 180 times greater (4.0GPa) strength than pure nickel.
It was found that there is a clear relationship between the interplanar distance and the strength of the multilayer material. A smaller interplanar distance made the dislocation movement more difficult and therefore increased the strength of the material. Professor Han, who led the research, commented, “the result is astounding as 0.00004% in weight of graphene increased the strength of the materials by hundreds of times” and “improvements based on this success, especially mass production with roll-to-roll process or metal sintering process in the production of ultra-high strength, lightweight parts for automobile and spacecraft, may become possible.” In addition, Professor Han mentioned that “the new material can be applied to coating materials for nuclear reactor construction or other structural materials requiring high reliability.”
The research project received support from National Research Foundation, Global Frontier Program, KAIST EEWS-KINC Program and KISTI Supercomputer and was a collaborative effort with KISTI (Korea Institute of Science and Technology Information), KBSI (Korea Basic Science Institute), Stanford University, and Columbia University.
A schematic diagram shows the structure of metal-graphene multi-layers. The metal-graphene multi-layered composite materials, containing a single-layered graphene, block the dislocation movement of graphene layers, resulting in a greater strength in the materials.
2013.08.23 View 16457 -
KAIST unveils foldable micro electric car, Armadillo-T
The small and light electric car completely folds in half when parking, making it a perfect fit for public or private transportation in an urban environment.
Looking for a parking space for hours at a busy shopping mall or being stuck on roads jammed with cars releasing large amounts of carbon dioxide are all-too-familiar scenes for city dwellers.
A group of researchers at the Korea Advanced Institute of Science and Technology (KAIST) recently developed a possible solution to such problems: a foldable, compact electric vehicle that can be utilized either as a personal car or part of the public transit system to connect major transportation routes within a city.
In-Soo Suh, associate professor of the Graduate School for Green Transportation at KAIST, and his research team introduced a prototype micro electric car called "Armadillo-T," whose design is based on a native animal of South America, the armadillo, a placental mammal with a leathery armor shell.
The research team imitated the animal"s distinctive protection characteristic of rolling up into a ball when facing with threat from predators. Just as armadillos hide themselves inside the shell, Armadillo-T tucks its rear body away, shrinking its original size of 2.8 meters (110 inches) down to almost half, 1.65 meters (65 inches), when folding.
Armadillo-T is a four-wheel-drive, all-electric car with two seats and four in-wheel motors. Since the motors are installed inside the wheels, and the 13.6 kWh capacity of lithium-ion battery pack is housed on the front side, the battery and motors do not have to change their positions when the car folds. This not only optimizes the energy efficiency but also provides stability and ample room to drivers and passengers.
Once folded, the small and light (weighs 450 kg) electric vehicle takes up only one-third of a 5-meter parking space, the standard parking size in Korea, allowing three of its kind to be parked. With a smartphone-interfaced remote control on the wheels, the vehicle can turn 360 degrees, enhancing drivers" convenience to park the car, even in an odd space in a parking lot, the corner of a building, for example.
Professor In-Soo Suh said, "I expect that people living in cities will eventually shift their preferences from bulky, petro-engine cars to smaller and lighter electric cars. Armadillo-T can be one of the alternatives city drivers can opt for. Particularly, this car is ideal for urban travels, including car-sharing and transit transfer, to offer major transportation links in a city. In addition to the urban application, local near-distance travels such as tourist zones or large buildings can be another example of application."
The concept car has loads of smart features on board, too: the cameras installed inside the car eliminate the need for side mirrors and increase the driver"s ability to see the car"s right and left side, thereby reducing blind spots. With a smartphone, the driver can control Armadillo-T and enable remote folding control. The car has a maximum speed of 60 km/h, and with a ten-minute fast charge, it can run up to 100 km.
Professor Suh explained that the concept of Armadillo-T was originally initiated in 2011 as he focused his research interest on the sub-A segment of personal mobility vehicles (PMVs), which are smaller and lighter than the current compact cars, as a new personalized transport mode.
"In coming years, we will see more mega-size cities established and face more serious environmental problems. Throughout the world, the aging population is rapidly growing as well. To cope with climate, energy, and limited petroleum resources, we really need to think outside the box, once again, to find more convenient and eco-friendly transportation, just as the Ford Model T did in the early 1920s. A further level of R&D, technical standards, and regulatory reviews are required to have these types of micro vehicles or PMVs on the market through test-bed evaluations, but we believe that Armadillo-T is an icon toward the future transport system with technology innovation."
The research project has been supported by the Korean government, the Ministry of Land, Infrastructure and Transport and the Korea Agency for Infrastructure Technology Advancement, since December 2012.Youtube Link: http://www.youtube.com/watch?v=8DoZH7Y-sR0
2013.08.21 View 15521 -
KAIST's HUBO Ready for DARPA's Robotics Challenge Trials
When walking on muddy or bumpy roads, the two arms of DRC-HUBO become extra legs, enabling stable and agile movements.
The Humanoid Robot Research Center (HUBO Lab, http://hubolab.kaist.ac.kr) at the Korea Advanced Institute of Science and Technology (KAIST) and Rainbow Co., a spin-off venture company of the university, unveiled a new model of HUBO that will be entered in an international robotics competition scheduled later this year.
The competition is hosted and sponsored by the US Defense Advanced Research Projects Agency (DARPA), which is called the DARPA Robotics Challenge (DRC). Kicked off in October 2012, the DRC’s goal is to spur the development of advanced robots that can assist humans in mitigating and recovering from future natural and man-made disasters.
KAIST’s humanoid robot, HUBO, was originally created by Jun-Ho Oh, a distinguished professor of the Department of Mechanical Engineering, in 2004. Since then, the robot has gone through technological advancements, with the latest version of HUBO II released in 2012. So far, 12 HUBOs have been exported for further studies in robotics to universities, research institutes, and private companies in the US, China, and Singapore.
In tandem with Rainbow Co. (www.rainbow-robot.com), Professor Oh and his research team recently developed DRC-HUBO, which will compete as Team DRC-HUBO led by Drexel University at the DRC trials to be held in December 2013. Team DRC-HUBO is consisted of KAIST and nine US institutions.
DRC-HUBO is designed to perform difficult but essential activities required when responding to disaster scenes. The robot will have to fulfill eight tasks assigned by the DRC at the upcoming event such as driving a utility vehicle, walking across rough terrain, climbing a ladder, and using hand tools.
Unlike the previous models of HUBO, DRC-HUBO boasts several distinctive, enhanced features. Chief among them is the way the robot interacts with the external environment. Without complex sensors installed throughout the body, DRC-HUBO can control each joint of the arms and legs in compliance with the dynamics dictated by the external environment.
For example, when DRC-HUBO is faced with a rock falling from above while climbing up a ladder, the robot’s arms and legs naturally give in to the force of external changes. Accordingly, as the robot dodges the rock, its body and joints smoothly sway to absorb shock so that the fingers can keep a tight grip on the ladder, and the feet are planted firmly on the rail of the ladder, not losing balance.
In addition, DRC-HUBO can switch from bipedal to quadrupedal walking and vice versa. This provides the robot with greater stability to walk on uneven terrain or to climb up a hill. The robot’s arms and legs are elongated to better meet the challenges demanded by the DRC competition. DRC-HUBO’s two arms swing back and forth to form legs when necessary, thereby walking freely backwards and forwards.
The robot has gotten stronger grip as well. The right hand has four fingers (with one triggering finger that operates independently from the other three fingers), and the left hand has three fingers. All three fingers on both hands are actuated synchronously for gripping. The fingers are sophisticated enough to steer the wheel of a vehicle or grab a ladder to climb up, and strong enough to hold 15 lbs in one hand.
“With a full 34 degrees of freedom (DOF), DRC-HUBO stands 4.7 ft tall and weighs 120 lbs. All in all, the robot has been improved and extensively refurbished from the past models of HUBOs to compete at the DRC Trials. It has better vision and coordination. The legs and arms have become stronger,” said Professor Oh.
“Although the robot is still a prototype, it has important capabilities that can be utilized in advancing humanoid robots in general. One example is the way its arms can be used as extra legs to support the robot’s body, offering more flexibility in providing aid to humans.”
2013.07.25 View 14729 -
A magnetic pen for smartphones adds another level of conveniences
Utilizing existing features on smartphones, the MagPen provides users with a compatible and simple input tool regardless of the type of phones they are using.
A doctoral candidate at the Korea Advanced Institute of Science and Technology (KAIST) developed a magnetically driven pen interface that works both on and around mobile devices. This interface, called the MagPen, can be used for any type of smartphones and tablet computers so long as they have magnetometers embedded in.
Advised by Professor Kwang-yun Wohn of the Graduate School of Culture Technology (GSCT) at KAIST, Sungjae Hwang, a Ph.D. student, created the MagPen in collaboration with Myung-Wook Ahn, a master"s student at the GSCT of KAIST, and Andrea Bianchi, a professor at Sungkyunkwan University.
Almost all mobile devices today provide location-based services, and magnetometers are incorporated in the integrated circuits of smartphones or tablet PCs, functioning as compasses. Taking advantage of built-in magnetometers, Hwang"s team came up with a technology that enabled an input tool for mobile devices such as a capacitive stylus pen to interact more sensitively and effectively with the devices" touch screen. Text and command entered by a stylus pen are expressed better on the screen of mobile devices than those done by human fingers.
The MagPen utilizes magnetometers equipped with smartphones, thus there is no need to build an additional sensing panel for a touchscreen as well as circuits, communication modules, or batteries for the pen. With an application installed on smartphones, it senses and analyzes the magnetic field produced by a permanent magnet embedded in a standard capacitive stylus pen.
Sungjae Hwang said, "Our technology is eco-friendly and very affordable because we are able to improve the expressiveness of the stylus pen without requiring additional hardware beyond those already installed on the current mobile devices. The technology allows smartphone users to enjoy added convenience while no wastes generated."
The MagPen detects the direction at which a stylus pen is pointing; selects colors by dragging the pen across smartphone bezel; identifies pens with different magnetic properties; recognizes pen-spinning gestures; and estimates the finger pressure applied to the pen.
Notably, with its spinning motion, the MagPen expands the scope of input gestures recognized by a stylus pen beyond its existing vocabularies of gestures and techniques such as titling, hovering, and varying pressures. The tip of the pen switches from a pointer to an eraser and vice versa when spinning. Or, it can choose the thickness of the lines drawn on a screen by spinning.
"It"s quite remarkable to see that the MagPen can understand spinning motion. It"s like the pen changes its living environment from two dimensions to three dimensions. This is the most creative characteristic of our technology," added Sungjae Hwang.
Hwang"s initial research result was first presented at the International Conference on Intelligent User Interfaces organized by the Association for Computing Machinery and held on March 19-22 in Santa Monica, the US.
In the next month of August, the research team will present a paper on the MagPen technology, entitled "MagPen: Magnetically Driven Pen Interaction On and Around Conventional Smartphones" and receive an Honorable Mention Award at the 15th International Conference on Human-Computer Interaction with Mobile Devices and Services (MobileHCI 2013) to be held in Germany.
In addition to the MagPen, Hwang and his team are conducting other projects to develop different types of magnetic gadgets (collectively called "MagGetz") that include the Magnetic Marionette, a magnetic cover for a smartphone, which offers augmented interactions with the phone, as well as magnetic widgets such as buttons and toggle interface.
Hwang has filed ten patents for the MagGetz technology.
Youtube Links: http://www.youtube.com/watch?v=NkPo2las7wc, http://www.youtube.com/watch?v=J9GtgyzoZmM
2013.07.25 View 11464 -
Thinking Out of the Box: KAIST Silicon Valley Innovation Platform
KAIST established a liaison office in San Jose, California, to support the entrepreneurship of KAIST graduates, students, and faculty who aspire to transform their innovative ideas into business.
The office, KAIST Silicon Valley Innovation Platform (SVIP), is located within the Korea Trade-Investment Promotion Agency (KOTRA) IT Center on North First Street in San Jose.
SVIP collects information and analyzes trends on emerging technologies; provides various educational programs on entrepreneurship and technology translation; offers opportunities to prospective entrepreneurs to engage with industry and research and government organizations; and assists Korean startups in accessing the US and North American market.
President Steve Kang attended the opening ceremony of the office on June 14th and encouraged KAIST alumni living in the US to share their ideas and technology innovations and transform them into business opportunities.
For more information, please contact Professor Soung-Hie Kim (seekim@business.kaist.ac.kr) from the Graduate School of Information and Media Management, KAIST.
2013.07.04 View 9025 -
KAIST Placed 3rd in the World's Best 100 Emerging Universities
The Times Higher Education (THE) published its world university rankings on June 20, 2013. It is a list of the best 100 universities whose histories are 50 years old or younger. KAIST was ranked 3rd in the list, two places up from the 5th last year. Forbes and Reuters carried a story on the top ten emerging universities out of the listed 100 institutions, highlighting strong showings in the Asian region. For the articles, please see the attached file (Forbes) or click the link (Reuters) below:
http://www.reuters.com/article/2013/06/19/education-university-rankings-idUSL2N0EU1HZ20130619
2013.06.22 View 7839
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Technology for Non-Breaking Smartphone Display Developed
High-strength plastic display has been developed by applying a glass-fiber fabric.
“Will bring about innovation to the field by replacing glass substrates”
It is now possible to manufacture non-breaking smartphone display. Heavy glass substrates of large-screen televisions will be replaced with light plastic films.
Professor Choon Sup Yoon from KAIST’s Department of Physics and KAIST Institute for Information Technology Convergence has developed the technology for high-strength plastic substrates to replace glass displays.
The plastic substrate created by Professor Yoon and his research team have greatly enhanced needed properties of heat resistance, transparency, flexibility, inner chemical capability, and tensile strength. Although the material retains flexibility as a native advantage of plastic film, its tensile strength is three times greater than that of normal glass, which is a degree similar to tempered glass. In addition, Professor Yoon’s substrate is as colorless and transparent as glass and resists heat up to 450℃, while its thermal expansivity is only 10% to 20% of existing plastics.
Glass substrates are currently used in practically every display such as mobile phone screens, televisions, and computer monitors for having smooth surface and satisfying basic conditions for display substrates. However, as glass substrates are heavy and easily broken, researchers studied colorless and transparent plastic polyimide films to replace glass substrates for their excellent thermal and chemical stability.
Nonetheless, colorless and transparent polyimide films do not have sufficient heat resistance and mechanical solidity. To resolve this problem, polyimide films are impregnated with glass-fiber fabrics, but it was far from commercialization as the impregnation exacerbates the roughness of surface and light transmittance. The roughness of the surface increases as the solvent evaporates in the impregnation process, resulting in surface roughness of around 0.4μm. The downturn in light transmittance is due to light scattering effect by the discording refractive index of polyimide film and glass-fiber fabric.
Professor Yoon’s research team resolved these issues by tuning the refractive indices of transparent polyimide film and glass-fiber fabric up to four decimal places, and by developing the technology of flattening the film’s surface roughness to a few nanometers. As a result, the research team achieved heat expansivity of 11ppm/℃, surface roughness of 0.9nm, tensile strength of 250MPa, bending curvature radius of 2mm, and light transmittance at 90% with a 110μm-thick glass-fiber fabric impregnated transparent polyimide film substrate.
“The developed substrate can not only replace the traditional glass substrate but also be applied as flexible display substrate,” said Professor Yoon in prospect, “it will bring about technological innovation in display industry as it can fundamentally resolve the issue of shattering mobile phone displays, reduce the weight and thickness of large-area televisions, and apply Roll to Roll process in display manufacture.”
Supported by the Ministry of Knowledge Economy for five years, the technology has applied for 3 patents and is in discussion for technology transfer with related business.
Figure 1. The according (left) and discording (right) refractive indices of glass-fiber fabric and polyimide film. The characters on the left are sharp and clear, but the characters on the right appear foggy.
Figure 2. Picture of the developed glass-fiber fabric
2013.06.09 View 9402